POLYCLONAL ANTIBODY DEVELOPMENT

A suitable mammal, such as a mouse, rabbit, sheep, or goat, is inoculated with a specially designed antigen (purified protein, synthetic peptides, organic compounds, or viral particles) to produce polyclonal antibodies (pAb). This causes the B-lymphocytes to produce IgG immunoglobulins that specifically target the antigen. The mammal's serum is used to purify this polyclonal IgG. In Western blots, ELISAs, IHCs, and other quantitative immunological tests, they can be employed as primary or secondary antibodies. Thanks to our collaborator's permission to use animal facilities for research, we are able to offer polyclonal antibody services in mice, rats, rabbits, chickens, goats, sheep, SPF animals, and other species. Antigen design, peptide synthesis, vaccination, purification, ELISA testing, and lyophilization are all included in our polyclonal antibody service package.

Main Features :
ELISA guarantee >1:40,000
WB positive guarantee for protein antigen.
Start as low as INR 90,000, deliver as fast as 80 days.
In-house antigen preparation, including peptide synthesis and gene to protein production service.
Several animals are available: Rabbits, Goats, Sheep, mice, rats and Guinea Pigs.
5 days express delivery to worldwide customers.

Polyclonal Antibody Production Packages

No:PPPAB01-WB Guaranteed Protein Polyclonal Antibody Package    

INR 105000

Antigen: protein( 3-5mg, >85%), customer to supply;
EDT: 3 months;
Service Contents: protein QC, 2 rabbits immunization, protein A/G purification, ELISA identification.
Guarantees:
ELISA >1:40,000; WB positive.
Deliverables:
1) 15-20mg protein A/G purified antibodies;
2) Pre-immune serum, 0.5 ml/rabbit; Immune serum, 0.5 ml/rabbit;
3) Project report (ELISA data+COA).

No:PPPAB02 Peptide Specific Polyclonal Antibody Package

INR 90800

Antigen: peptide(10mg, ≥85%), Priveel Custom Peptides to synthesize;
EDT: as fast as 90 Days;
Service Contents: peptide synthesis & KLH conjugation & BSA conjugation, 2 rabbits immunization, antigen affinity purification, ELISA identification.
Guarantees:
ELISA >1:40,000.
Deliverables:
1) 3-5mg affinity purified antibodies;
2) 1mg peptide, 0.1mg peptide-BSA;
3) Pre-immune serum, 0.5 ml/rabbit; Immune serum, 0.5 ml/rabbit;
4) Project report (ELISA data+COA). 

No:PPPAB03 Protein Polyclonal Antibody Package

INR 280000

Antigen: 3-5mg protein(>85%,>0.5mg/ml), Priveel Custom Peptides to produce;
EDT: 4-5 months;
Service Contents: subcloning, protein expression and purification, 2 rabbits immunization, protein A/G purification, ELISA identification
*Customer to provide expression plasmid or Priveel Custom Peptides can start with de novo gene synthesis (INR 75/bp).
*The package price is based on friendly target protein for expression.
Guarantees:
ELISA >1:40,000; WB positive.
Deliverables:
1) 15-25 mg protein A/G purified antibodies;
2) 0.5ml Pre-immune serum & 0.5ml immune serum/rabbit;
3) 0.1mg protein with tag (>85%);
4) Project report (ELISA data+COA).

No:PPPAB04 Phospho Specific Polyclonal Antibody Package

INR 165000

Antigen: phospho peptide(15mg, >85%), non-phospho peptide(10mg, >85%), Priveel Custom Peptides to synthesize;
EDT: 4 months;
Service Contents: peptide synthesis & KLH conjugation & BSA conjugation, 4 rabbits immunization, antigen affinity purification; ELISA identification.
Guarantees:
ELISA >1:40,000;
DB result - no cross reaction.
Deliverables:
1) 3-5 mg affinity purified phospho antibodies;
2) 3-5 mg affinity purified non-phospho antibodies;
3) 1 mg of phospho & non-phospho peptides; 0.1mg BSA conjugated phospho & non-phospho peptides;
4) 0.5ml Pre-immune serum & 0.5ml immune serum/rabbit;
5) Project report (ELISA + DB data+COA). 

No:PPPAB05 Formyl Specific Polyclonal Antibody Package

INR 250000

Antigen: formyl peptide(15mg, >85%), non-formyl peptide(10mg, >85%), Priveel Custom Peptides to synthesize;
EDT: 4 months;
Service Contents: peptide synthesis & KLH conjugation & BSA conjugation, 4 rabbits immunization, antigen affinity purification, ELISA and DB identification.
Guarantees:
ELISA >1:40,000;
DB result, no cross reaction.
Deliverables:
1) 3-5mg affinity purified formyl antibodies;3-5 mg affinity purified non-formyl antibodies;
2) 1 mg of formyl & non-formyl peptides;
3) 0.1mg BSA conjugated formyl & non-formyl peptides;
4) 0.5ml Pre-immune serum & 0.5ml immune serum/rabbit;
5) Project report (ELISA + DB data+COA).

No:PPPAB06 Methyl Specific Polyclonal Antibody Package

INR 205000

Antigen: methyl peptide(15mg, >85%), non-methyl peptide(10mg, >85%), Priveel Custom Peptides to synthesize;
EDT: 4 months;
Service Contents: peptide synthesis & KLH conjugation & BSA conjugation, 4 rabbits immunization, antigen affinity purification, ELISA and DB identification.
Guarantees:
ELISA >1:40,000;(methyl specific antibody); DB result - no cross reaction.
Deliverables:
1) 3-5 mg affinity purified methyl antibodies;
2) 3-5 mg affinity purified non-methyl antibodies;
3) 1 mg of methy & non-methyl peptides;
4) 0.1mg BSA conjugated methy & non-methyl peptides;
5) 0.5ml Pre-immune serum & 0.5ml immune serum/rabbit;
6) Project report (ELISA + DB data+COA). 

Our Contacts

Address:

Priveel Custom Peptides
RMZ Milenia Business Park,
Phase 2, Campus 3B, 
Dr. M.G.R Road, Kadanchavady,
Chennai, Tamil Nadu 600096

Phone:

+91 9123531574

E-mail:

getinfo@priveelpeptides.com

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